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The aim of the studies was to compare the Ht values and the content of Hb in blood, total protein. Na, K, Ca, inorganic P and Mg in blood serum and bone tissue in lambs born in different seasons. A higher (P≤0.05) Ht value, K and Ca content, and a lower total protein, Na, inorganic P and Mg content were found in the blood of lambs born in autumn. A higher (P≤0.05) content of Ca and Mg, but a lower...
The aim of these studies was to determine the performances of five commercial ELISA kits in the detection of BLV antibodies in serum samples. Different variants of ELI SA methods (indirect, blocking and double-well) were employed for these kits. Comparative investigations using 428 serum samples showed 418 consistent results (97.7%). Ten results (2.3%) proved to be inconsistent including 7 (1.6%)...
The applicability of the polymerase chain reaction (PCR) for the detection of proviral DNA of BLV in newborn calves was tested. Out of 22 calves from BLV infected mothers, studied during first 3 weeks after birth, 13 were serologically positive by ELISA. However, only 3 calves were positive for BL V provirus in their blood leukocytes’ DNA. Since the serological test can not discriminate active infection...
The results of PCR were compared with the results of virus isolation test. 44 bovine semen samples were tested. The presence of BHV 1 was demonstrated with the virus isolation test in 6 samples of semen. PCR with external primers detected BHV 1 in 8 samples, while in nested PCR positive amplification was observed in 21 samples. The sensitivity of PCR with external primers and nested PCR were 0.6 and...
The effects of iv infusion of 7.2% NaCl (HSS, 4 ml/kg; n=6) or 7.2% NaCl in 6% dextran 70 (HSD, 4 ml/kg; n=6) followed by administration of 0.9% NaCl (ISS, 32 ml/kg) on sodium (Na), chloride (Cl), potassium (K) serum concentrations, osmolality (Osm), total protein content (TP), packed cell volume (PCV), haemoglobin concentration (Hb) and red blood cell count (RBC) were evaluated in twelve healthy...
Evaluation of the immunogenicity of the vaccine against respiratory tract infections in calves caused by Pasteurella multocida, and prepared from antigen of a local strain of serotype 3 with oil adjuvant addition, was the object of the prescnt studies. Two experiments were carried out using calves. Vaccine was given them at a dose of 2 ml i.m., twice every 14 days. In experiment 1, sera were obtained...
The aim of this studies was the genetic analysis of Polish SVDV isolates from 1972-73. The direct nucleotide sequencing technique of PCR products from the 1D (VP1) structural polipeptyde coding region was applied. The genetic differences between Polish isolates and other European and Japanese ones from 1966-1994 were determined by comparing their nucleotide sequences. No considerable genetic divergences...
Male Wistar rats received orally a solution of cadmium chloride at a dose corresponding to 10 mg of cadmium/kg of diet. Rats were killed at 7, 14, 21 and 28 days of cadmium administration and 7 days postdosing. Results showed that the cadmium intoxication affected renal and hepatic glutathione content and superoxide dismutase in a dose-dependent manner. Changes in glutathione S-transferase activity...
The subjects were 13 horses, of which 5 clinically healthy ones were infected intranasally and intraconjunctivally with a suspension of two subtypes of the influenza virus: A/equi/1 and A/equi/2, and 3 were control animals. The remaining 5 horses became ill in a natural way. In the early phase of injection, the haematological determinations showed in the experimental horses a decrease in the lcucocyte,...
The subjects were 8 clinically healthy Polish Primitive Horses at 2-9 years of age. The horses were given E.coli LPS four times, in a dose of 0.1 μg/kg.b.w.; 3 times at 24 hour intervals (LPS-1 , LPS-2, LPS-3 ), and one week after the last infusion (LPS-4). The result analysis indicated that the applied endotoxin dose caused development of tolerance mechanisms related to the platelet count. This phenomenon...
Studies on effects of pasteurisation of meat with spiked hormones at residue levels have been made. Beef and poultry meat samples which contained diethylstilbestrol and 19-nortestosterone were heated at 80°C for 20 minutes. The hormones were quantified by high-performance liquid chromatography with UV detection on raw and pasteurised meat samples. Diethylstilbestrol is stable under these conditions...
During 19-day study with broiler poultry, broiler chicks were fed diet containing clinoptilolite as supplement at the concentrations of 3% and 5%. At the same time, the following parameters were observed: activity of alkaline phosphatase (ALP) and body weight of broiler chicks. ALP activity in broiler chicks (groups G4, G5, and G6) fed aflatoxin B₁ was enhanced during all days of the observation in...
After the intramuscular administration of Engemycin L.A. inj. ad us. vet. (0.8 ml. 10kg⁻¹) to seven ewes for four consecutive days, the milk samples were collected at every 12 h of the experiment, from 0 to 166 h. The milk samples were analysed by the high performance liquid chromatography (HPLC) method. Twelve hours after the beginning of the experiment, all milk samples were positive for the presence...
The prevalence of bovine viral diarrhea (BVD) virus infection on the basis of serological testing of cattle has been evaluated. Blood samples were analyzed in micro-seroneutralization test for the presence of BVD virus neutralizing antibodies against 1000 TCID₅₀ of NADL reference strain. Neutralizing antibodies were found in 83 per cent of animals tested. The percentage of seropositive animals was...
RT-PCR was evaluated as a diagnostic tool for BVDY detection. First, five sets of primers were checked for their diagnostic ability to amplify the number of field isolates. Only primers within the 5'UTR region amplified all field isolates as well as all reference laboratory strains. Next, sensitivity of the test was evaluated. This was equal to 1 TCID₅₀ of the virus. lt was concluded that RT-PCR can...
Decontamination effectiveness of ammonium-ferric(lll)-hexacyanoferrate(II) (AFCF) in reducing the radiocaesium transfer into dairy goat milk was examined. The most effective reduction of radioactivity burden in milk was found in animals treated with AFCF as early as the first day of radionuclide contamination. Milk radioactivity concentrations in goats treated simultaneously with ¹³⁷CsCI and hexacyanoferrate...
The study was performed on pregnant ewes and their progeny. The experimental group of ewes was given injections of 5 mg selenium in the form of sodium selenite. The sodium selenite s.c. injections resulted in monre than a triple, significant increase in the selenium level in ewes, at the first month as compared to the initial concentration of 0.42±0.11 to 1.38±0.21 μmol/l. Also in lambs a marked increase...
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